Department of biophysics Palacky University

HomeResearch topicsCollaborationPublications

A new 4-(pyridinyl)-4H-benzo[g]chromene-5,10-dione ruthenium(II) complex inducing senescence in 518A2 melanoma cells.

Autor: Gold M., Mujahid J., Ahmed K., Kostrhunova H., Kasparkova J., Brabec V., Biersack B., Schober R.Published: Journal of Biological Inorganic Chemistry 24(5), 647-657Year: 2019

2-Amino-5,10-dihydro-5,10-dioxo-4(pyridine-3-yl)-4H-benzo[g]chromene-3-carbonitrile 5, a cytotoxic lawsone derivative, was reacted with [Ru(p-cymene)Cl2]2 to afford a new Ru(II) ‘piano stool’ complex 6 which differed from its ligand 5 by a greater selectivity for highly invasive 518A2 melanoma cells over human dermal fibroblasts in MTT cytotoxicity assays, and by inducing senescence rather than apoptosis in the former. DNA is a likely cellular target of complex 6 as it bound, presumably non-covalently, to linear and circular double-stranded DNA in vitro and as ruthenium was found in the lysate of nuclei of treated 518A2 melanoma cells. It also caused a fivefold increase of reactive oxygen species in these cells, originating from a more persistent redox cycling as visualized by cyclic voltammetry.

Department of Biophysics

Faculty of Science

Palacký University in Olomouc, Czech Republic

© 2011 Department of Biophysics
Created: Webdesign Olomouc |