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Alternative electron transport mediated by flavodiiron proteins is operational in organisms from cyanobacteria up to gymnosperms.

Autoři: Ilik P., Pavlovič A., Kouril R., Alboresi A., Morosinotto T., Allahverdieyeva Y., Aro E.-M., Yamamoto H., Shikanai T.Publikováno : New Phytologist 214(3), 967-972Rok: 2017

Photo-reduction of O2 to water mediated by flavodiiron proteins (FDPs) represents a safety valve for photosynthetic electron transport chain in fluctuating light. So far, the FDP-mediated O2 photo-reduction has been evidenced only in cyanobacteria and the moss Physcomitrella, however, a recent phylogenetic analysis of transcriptomes of photosynthetic organisms has revealed the presence of FDP genes also in several non-flowering plant groups. What remains to be clarified is whether the FDP–dependent O2 photo-reduction is actually operational in these organisms.
We have established a simple method for the monitoring of FDP-mediated O2 photo-reduction, based on the measurement of redox kinetics of P700 (the electron donor of photosystem I) upon dark-to-light transition. The O2 photo-reduction is manifested as a fast re-oxidation of P700. The validity of the method was verified by experiments with transgenic organisms, namely FDP knock-out mutants of Synechocystis and Physcomitrella and transgenic Arabidopsis plants expressing FDPs from Physcomitrella.
We observed the fast P700 re-oxidation in representatives of all green plant groups excluding angiosperms. Our results provide strong evidence that the FDP-mediated O2 photo-reduction is functional in all non-flowering green plant groups. This finding suggests a major change in the strategy of photosynthetic regulation during the evolution of angiosperms.


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