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Transitions in the thylakoid membranes of barley leaves studied by chlorophyll fluorescence temperature curve.

Autoři: Nauš J., Dvořák L., Kuropatwa R., Mašláň M.Publikováno : Photosynthetica 27(4), 563-570Rok: 1992

Chlorophyll (Chl) fluorescence temperature curve (FTC) of primary leaves of spring barley (Hordeum vulgare L.), cultivated under a low irradiance, was studied at low actinic radiation excitation (436 nm, 2 W m-2) under a heating regime consisting of a linear increase of temperature (0.083 °C s-1), followed by a period of constant temperature. After prompt cooling of the sample to 77 K, Chl fluorescence spectra at defined points of the temperature regime were measured. Two distinct temperature and kinetic regions expressed by two discernible temperature maxima were observed for this type of the FTC. Two triggering temperatures of successive irreversible changes are postulated at 45-48 °C and 53-55 °C temperature intervals, respectively; they may correlate with the heat inactivation of photosystem 2 and with changes of the chloroplast and cytoplasmic membrane integrity.

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